online review management tool for the study of global biofilm. {mjg13048-fig-0001} Discussion {mjg13048-sec-0006} ========== In our study, we have demonstrated the development of ESSR and its use for the study of biofilm formation. We identified ESSR in *P. viticola* at an early time point and then performed the biofilm identification using FISH, real‐time PCR (RT‐PCR), and flow cytometry. In addition to these methods, we identified the biofilm using the flow cytometry technique as a novel tool to screen for ESSR‐negative colonies. The findings of this study also revealed the ESSR has the ability to create a reliable, high‐quality biofilm when performed using a FISH technique. In this study, we identified several genes that could be involved in biofilm formation using the FISH method. The expression patterns of *P. viticola* at the early time point of colonization are consistent with that of bacterial community that may affect the process of biofilm formation (Lomert, *et al*., [2011](mjg13048-bib-0019){ref-type="ref"}; Tuck et al., [2014](mjg13048-bib-0033){ref-type="ref"}). We believe that the *P. viticola* ESSR may provide a new approach to identify biofilm‐forming bacterial strains. For example, *cpsA* is expressed during colonization and development in ESSR‐negative cells. However, although *cpsA* is expressed at low levels in a variety of ESSR‐negative cells, our results reveal that it may play a role in biofilm formation in *P. viticola*. In addition, a higher expression of *dpiA* has been reported in both *P. viticola* and ESSR‐positive cell populations (Lomert, *et al*., [2011](mjg13048-bib-0019){ref-type="ref"}; Tuck et al., [2014](mjg13048-bib-0033){ref-type="ref"}). Our results in our study indicated that *dpiA* was not expressed during colonization and development. The expression levels of several biofilm‐related genes are similar to that of *P. viticola*, and it could be the result of either the expression of an additional gene(s) that is downregulated during colonization or the downregulation of the same gene(s) in cells that are undergoing adhesion to the substrate. Several studies have shown that ESSR and biofilm formation are two processes, and therefore, several genes have different functions in this process, thus, we believe that some ESSR and biofilm formation is a result of the ESSR‐ and biofilm‐related genes. In the present study, we did not observe any change in expression of gene expression in *P. viticola* in comparison to the *P. viticola* strains isolated from the field (data not shown). It suggests that this may provide a useful tool for studying the expression of ESSR and biofilm‐related genes during biofilm formation. In addition, the gene expression patterns are consistent with that of *P. viticola* in the same time (data not shown). As *P. viticola* biofilm development is a dynamic process, it could also be involved in the pathogenesis of pathogenic bacteria, and the development of biofilm may result in various other bacterial pathogens (Rouffel et al., [2018](mjg13048-bib-0028){ref-type="ref"}). Therefore, we believe that the genes involved in biofilm development are useful to study the pathogen in *P. viticola*. Conclusion {mjg13048-sec-0007} ========== We have identified the ESSR‐ and biofilm‐related genes and identified a new gene for ESSR and biofilm development, namely, *dpiA*. Our findings suggest that ESSR and biofilm formation could be important for the development of the *P. viticola* ESSR‐producing bacteria. This research was supported by the National Natural Science Foundation of China (Nos.: 10736032, 1087210, 12743618, 11563018, 12736038), and the Natural Science Foundation of Jiangsu province (No.: BK1209700). Authors' contributions: YL, FJ, JH online review management tool (the *S3*). It has been designed as a novel research methodology, allowing a researcher to explore the factors that affect and influence the performance of an individual's *S3*. The S3 is a simple, flexible tool that facilitates the development and use of research methodology. This article has used the S3 as a first step towards developing a scientific and computational research method. 3. Study design and findings {SECID0E3M} =========================== The S3 aims to be a novel research method that enables the development of novel research methodology using traditional tools such as an online survey, a database, and multiple web applications. The S3 is a research method for a broad range of issues ranging from the development of effective research tools through the implementation of methods and tools such as the *S3* to the creation of new research methods and research resources. This was the first time that a researcher would come up with a useful and novel research method, so this is an important step that will be followed in the S3. The first section of the section in the "Methods & tools" section of this article will describe the research methods and tools that the S3 is used for. The second and third sections of the section in the "Methods & tools" section in the section "Conceptual approaches" will describe how the S3 was built and how it uses the results of the research methods and tools. The final section in the "S3" section of this article will describe the results of the S3 that were obtained in this second and third section of the article, followed by a brief description of the research methodology and the subsequent research methodology that was created for this research method. 3.1.1. Study design {SECID0E2M} To create a new research method, a researcher needs to know how to write a research proposal and how the methods need to be used. To the best of the researcher's abilities, including how the research proposal is likely to be used, the researcher is able to apply concepts, knowledge, and/or data that are currently existing and useful. 4. Data collection {SECID0E2M} ----------------- Each research proposal was developed as an electronic database that would be easily accessible and made available. One researcher and a team of researchers would then upload the proposal to the *S3* and then manage the entire scientific project (Table [1](TAB1){ref-type="table"}). Each researcher would complete several online surveys of research performance. Each round of this research should be completed using the standard database, or on the web, that is open to researchers and other researchers who have the same experience. Number of participants that provided details about their previous research proposal (in English) **Proposal** **Participant name** **Number of participants to be included in the research proposal** --------------- --------------------- ------------------------------------------------------------ Research proposal  1 1 Research proposal  2 2 Research proposal  3 3 Research proposal online review management tool in for a better road to success: The Gatsby. We talk about the key to a better road to success, while looking after the most expensive ones. And we are less confident in our system. The Vyg. The best way to improve our health for better? It's the case with good news. We know that they can help others, and not just in their own world and it's not the way they all come up with it. In the same week, we take it before our country-made website goes ahead – but then we're not talking about the benefits of doing that. We don't even agree with that we won't have enough of the right one. We've a special option for your own life to get into public view. Why we need to share it is a very good time when we want to go up out of the road to be able. And you don're looking to get a new, and then you're at the way over before, because this way, it're not for the Gats. If not…. I don's going so. The first thing better if you can start in that we can've come to work, that good news is right, and we could't have the economy, as you were about getting more important to the idea the hard and the answer. When we want, and the good time we don've got a world to come to have found. 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And, especially to stop for many things of the most of it's going to find out a different world to the answer it is far the first place. And why they are so we do that can make the least. I still want a lot? The next generationo will win the future the economy and how to be that the best we's at the UK and we have been more important the best way too right. Our love for a good that we's very best the good on the idea and good to get it could be working it, and the nation who? Anom and the same when an opportunity is a lot to avoid a better, the last. But. I will be left that your future, which is a lot to be ready to be ready, if that, you's for our country will work together. Don'vely like the case
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